Journal: PLOS ONE
Article Title: Influence of controlled environmental conditions in potential salivary ocular pain biomarkers for enhancing the assessment of ocular pain
doi: 10.1371/journal.pone.0296764
Figure Lengend Snippet: Bland-Altman graphs showing the intersession reproducibility for each biomarker: (A) Cortisol (B) sAA, (C) Testosterone, (D) sIgA, and (E) sTNFαRII. The solid lines represent the upper and the lower LoA (limits of agreement): crude 95%. LoA are depicted in A and the 95% back transformed LoA after the 10 base log-transformation are shown as functions of the mean of the two measurements in the remaining (X denotes the corresponding biomarker mean). Dashed line represents the mean difference value between 2nd and 1 st salivary collections, and shaded area the magnitude between this mean difference value and zero.
Article Snippet: Cortisol, α-amylase (sAA), secretory IgA (sIgA), testosterone, and soluble fraction of TNFα receptor II (sTNFαRII) were analyzed in salivary samples by enzyme-linked immunosorbent assay (ELISA) using the commercially available kits: Cortisol (DRG® Salivary Cortisol ELISA, DRG® Instruments GmbH, Marburg, Germany), testosterone (DRG® Salivary Testosterone ELISA, DRG Instruments GmbH), sAA (DRG Salivary Alpha Amylase ELISA, DRG Instruments GmbH), sTNFαRII (Quantikine®, Human sTNF RII/TNFRSF1B Immunoassay, R&D Systems, Minneapolis, MN, USA) and sIgA (Salimetrics® Salivary Secretory IgA ELISA, Pennsylvania, USA).
Techniques: Biomarker Discovery, Transformation Assay